Home » speciality Dyes and Biological Stains » Giemsa Stain Powder
Giemsa stain was a name adopted from a German chemist scientist, Gustav Giemsa, for his application of a combination of reagents in demonstrating the presence of parasites in malaria. Giemsa stain is a neutral stain made up of a mixture of oxidized Methylene blue, Azure, and Eosin Y. It is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of Malaria, spirochetes and other blood parasites. It is also used to visualize chromosomes and to identify mast cells.
PRODUCT SPECIFICATIONS | |
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PRODUCT NAME | GIEMSA STAIN POWDER |
SYNONYMS | Mixture of Azure II Eosinate & Methylene Blue |
CAS No | 51811-82-6 |
Molecular Weight | 291.80 |
SR. No | Criteria | Limit/Specification |
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1 | APPEARANCE | Dark Green to black crystal or powder |
2 | Melting Point | 300 °C (lit.) |
3 | SOLUBILITY | 10 mg soluble in 10 ml of Methanol to give clear blue solution with greenish fluorescence. |
4 | ABSORBANCE | NLT 0.6 (Wavelength 650-656 nm, c=0.005 g/l in methanol) |
5 | ABSORBANCE | NLT 0.3 (Wavelength 520-526 nm, c=0.005 g/l in methanol) |
6 | ABSORBANCE | NLT 0.3 (Wavelength 288-294 nm, c=0.005 g/l in methanol) |
7 | ABSORBANCE | NLT 0.2%(Wavelength 243-249 nm, c=0.005 g/l in methanol) |
8 | LOSS ON DRYING (at 110°C, 1 hr.) |
NMT 8.0% |