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What is Acid Red 94/Rose Bengal
Acid Red 94 or Rose Bengal is an organic compound and belongs to the class of xanthenes. In 1882, Swiss chemist Robert Ghnem synthesized rose bengal as a  4,5,6,7-tetrachloro 2′,4′,5′,7′-tetraiodofluorescein analogue. It is a commonly used dye for detecting damage to the ocular surface epithelium in ocular surface diseases like dry eye and herpetic keratitis.
Macsen Laboratories is the leading manufacturer & supplier of Rose Bengal with ISO certification.
| PRODUCT SPECIFICATIONS | |
|---|---|
| Name of Product | Rose bengal disodium salt |
| IUPAC Name | disodium;4,5,6,7-tetrachloro-2',4',5',7'-tetraiodo-3-oxospiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate |
| Synonyms | Rose Bengal sodium salt; Xantryl; Food Red No. 105, sodium salt; PV 10; C.I. Acid Red 94; Rosa Bengala; Rosa del Bengala; Rose Bengale |
| CAS No | 632-69-9 |
| Molecular Formula | C20H2Cl4I4Na2O5 |
| Molecular Weight | 1017.6 g/mol |
| Other available variants | Rose Bengal Sodium (11121-48-5) Rose Bengal Lactone (4159-77-7) |
| Pubchem CID | 69438 |
| Pubchem SID | 469688486 |
Chemical Properties
| SR. No | Criteria | Limit/Specification |
|---|---|---|
| 1 | Appearance (Form) | Solid powder |
| 2 | Appearance (Color) | Dark Violet |
| 3 | λmax | 549 nm |
| 4 | Storage temp. | Room temp. |
Uses
- It has been used as a diagnostic agent for more than 50 years to identify liver and eye cancer.
- Its sodium salt is frequently employed in eye drops to color-code injured conjunctival and corneal cells, helping to identify eye injury.
- Clinical trials for melanoma, breast cancer, and neuroendocrine tumours are currently being conducted with PV-10 (an injectable version of rose bengal).
- Additionally, studies on rose bengal have revealed that it can treat colon cancer and stop the growth and spread of ovarian cancer.
- It is being investigated as a potential tool for making nano sutures.
- Further, the stain is used to prepare foraminifera for microscopic examination, enabling the differentiation between forms that were alive or dead at the time of collecting.
FAQs
Q. What is Rose Bengal test?
The Rose Bengal test (RBT) is a rapid slide-type agglutination assay performed with a stained B. abortus suspension at pH 3.6–3.7 for the serological diagnosis of brucellosis.
Q. What is the difference between Rose Bengal stain and fluorescein?
Rose Bengal is actually a derivative of fluorescein, however, unlike fluorescein it is not a vital dye. Fluorescein staining manifests whenever there is disruption of cell-cell junctions whereas rose bengal staining ensues whenever there is deficiency of preocular tear film protection. Fluorescein is distinguishable from rose bengal by its absence of inherent toxicity and photodynamic activity.
Resources
Biological Stains | Classification, Examples & Uses