Giemsa Stain Powder (51811-82-6)

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Giemsa stain was a name adopted from a German chemist scientist, Gustav Giemsa, for his application of a combination of reagents in demonstrating the presence of parasites in malaria. Giemsa stain is a neutral stain made up of a mixture of oxidized Methylene blue, Azure, and Eosin Y. It is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of Malaria, spirochetes and other blood parasites. It is also used to visualize chromosomes and to identify mast cells.

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PRODUCT SPECIFICATIONS
PRODUCT NAME GIEMSA STAIN POWDER
SYNONYMS Mixture of Azure II Eosinate & Methylene Blue
CAS No 51811-82-6
Molecular Weight 291.80

SPECIFICATIONS

SR. No Criteria Limit/Specification
1 APPEARANCE Dark Green to black crystal or powder
2 Melting Point 300 °C (lit.)
3 SOLUBILITY 10 mg soluble in 10 ml of Methanol to give clear blue solution with greenish fluorescence.
4 ABSORBANCE NLT 0.6 (Wavelength 650-656 nm, c=0.005 g/l in methanol)
5 ABSORBANCE NLT 0.3 (Wavelength 520-526 nm, c=0.005 g/l in methanol)
6 ABSORBANCE NLT 0.3 (Wavelength 288-294 nm, c=0.005 g/l in methanol)
7 ABSORBANCE NLT 0.2%(Wavelength 243-249 nm, c=0.005 g/l in methanol)
8 LOSS ON DRYING
(at 110°C, 1 hr.)
NMT 8.0%
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    Resources 

    Giemsa Stain | Composition, Principle, Procedure & Uses

    Biological Stains | Classification, Examples & Uses

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