Home » speciality Dyes and Biological Stains » Giemsa Stain Powder
Giemsa stain was a name adopted from a German chemist scientist, Gustav Giemsa, for his application of a combination of reagents in demonstrating the presence of parasites in malaria. Giemsa stain is a neutral stain made up of a mixture of oxidized Methylene blue, Azure, and Eosin Y. It is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of Malaria, spirochetes and other blood parasites. It is also used to visualize chromosomes and to identify mast cells.
| PRODUCT SPECIFICATIONS | |
|---|---|
| PRODUCT NAME | GIEMSA STAIN POWDER |
| SYNONYMS | Mixture of Azure II Eosinate & Methylene Blue |
| CAS No | 51811-82-6 |
| Molecular Weight | 291.80 |
| SR. No | Criteria | Limit/Specification |
|---|---|---|
| 1 | APPEARANCE | Dark Green to black crystal or powder |
| 2 | Melting Point | 300 °C (lit.) |
| 3 | SOLUBILITY | 10 mg soluble in 10 ml of Methanol to give clear blue solution with greenish fluorescence. |
| 4 | ABSORBANCE | NLT 0.6 (Wavelength 650-656 nm, c=0.005 g/l in methanol) |
| 5 | ABSORBANCE | NLT 0.3 (Wavelength 520-526 nm, c=0.005 g/l in methanol) |
| 6 | ABSORBANCE | NLT 0.3 (Wavelength 288-294 nm, c=0.005 g/l in methanol) |
| 7 | ABSORBANCE | NLT 0.2%(Wavelength 243-249 nm, c=0.005 g/l in methanol) |
| 8 | LOSS ON DRYING (at 110°C, 1 hr.) |
NMT 8.0% |