Home »Speciality Dyes and Biological Stains » Bromothymol Blue
What is Bromothymol Blue
Bromothymol blue belongs to the family of 2,1-benzoxothioles. It is an organobromine compound which acts as a weak acid in a solution, however, it can exhibit both acid or base form, depending on the pH of the solution.
In addition to acting as a dye and a two-color indicator, it can also serve as an acid-base indicator owing to its property of being yellow in acidic solutions, blue in basic solutions and green in neutral solution.
Macsen Laboratories is the leading manufacturer & supplier of Bromothymol Blue Dye with ISO certification.
| PRODUCT SPECIFICATIONS | |
|---|---|
| Name of Product | Bromothymol Blue |
| IUPAC Name | 2-bromo-4-[3-(3-bromo-4-hydroxy-2-methyl-5-propan-2-ylphenyl)-1,1-dioxo-2,1λ6-benzoxathiol-3-yl]-3-methyl-6-propan-2-ylphenol |
| Synonyms | Dibromothymolsulfophthalein; 3,3'-Dibromothymolsulfonphthalein; Bromthymolblau; Azul de bromotimol; Blu di bromotimolo; Bleu de bromothymol |
| CAS No | 76-59-5 |
| Molecular Formula | C27H28Br2O5S |
| Molecular Weight | 624.4 g/mol |
| Pubchem CID | 6450 |
| Pubchem SID | 469688485 |
Chemical Properties
| SR. No | Criteria | Limit/Specification |
|---|---|---|
| 1 | Appearance (Form) | Solid powder |
| 2 | Appearance (Color) | White to cream-coloured |
| 3 | Melting point | 201.0 °C |
| 4 | Density | 1.25 g/cm3 |
| 5 | Solubility | Sparingly soluble in water |
| 6 | Acidity (pKa) | 7.0 |
| 7 | λmax | 420 nm |
| 8 | Storage temp. | Room temp. |
Uses
- Bromothymol blue can be used to investigate photosynthetic processes or as a biomarker of respiratory activities.
- It serves as a pH indicator in the range from 6.0 to 7.6 because of the precise color transition at neutrality.
- In conjunction with phenol red, it has been used to monitor the asparaginase enzyme activity in fungi.
- It is used as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
- It is also used in obstetrics for detecting premature rupture of membranes.
FAQs
Q. What is the color of Bromothymol Blue in acid, base and neutral mediums?
Bromothymol Blue shows yellow color in acidic solutions, blue color in basic solutions and green color in neutral solutions respectively.
Q. Is bromothymol blue toxic?
Bromothymol blue is harmful if inhaled and may cause moderate respiratory tract irritation. Also, It is very dangerous if swallowed/ingested and cause gastrointestinal irritation with nausea, vomiting and diarrhea.
Q. Where to buy bromothymol blue?
Macsen Labs is a leading manufacturer and supplier of bromothymol blue with ISO certification.
Q. What is the pKa of bromothymol blue?
The pKa of Bromothymol Blue is approximately 7.0 to 7.2 at 25°C.
Buy the best quality Bromothymol Blue Dye through Macsen Laboratories. For buying, send us an enquiry-