Home » Speciality Dyes and Biological Stains » Auramine O
What is Auramine O?
Auramine O is an aniline dye and is obtained by combining 4,4′-carbonimidoylbis(N,N-dimethylaniline) with one molar equivalent of hydrogen chloride. It is a diarylmethane dye and functions as both a fluorochrome and a histology stain.
Macsen Laboratories is the leading manufacturer and supplier of Auramine O with ISO certification.
| PRODUCT SPECIFICATIONS | |
|---|---|
| Name of Product | Auramine O |
| IUPAC Name | 4-[4-(dimethylamino)benzenecarboximidoyl]-N,N-dimethylaniline;hydrochloride |
| Synonyms | Auramine hydrochloride; Basic yellow 2; Pyocatanium aureum; Aizen auramine; Pyoktanin yellow; Canary yellow, pyoktanin; Auramin; Benzophenoneidum; Calcozine Yellow OX |
| CAS No | 2465-27-2 |
| Molecular Formula | C17H21N3.HCl |
| Molecular Weight | 303.8 g/mol |
| Color Index No | 41000 |
| Pubchem CID | 17170 |
| Pubchem SID | 475523901 |
CHEMICAL PROPERTIES
| SR. No | Criteria | Limit/Specification |
|---|---|---|
| 1 | Appearance (form) | Solid Powder or Flakes |
| 2 | Appearance (Color) | Yellow |
| 3 | Melting Point | >250 °C (dec) |
| 4 | Solubility | 10 mg/ml in Water 60 mg/ml in Ethylene glycol methyl ether 20 mg/ml in Ethanol |
| 5 | Solubility (Color) | Clear to hazy, Yellow to very deep yellow |
| 6 | Storage | Room Temperature |
Uses
- In biology, Auramine O is used in combination with Rhodamine B as auramine-rhodamine stain to stain acid-fast organisms, e.g. Mycobacterium, where it forms a complex with the mycolic acid found in its cell wall.
- It can be utilized in the dying of acrylic fibres, silk, and cotton, as well as leather, paper, hemp, and viscose.
- It can also be used in direct printing and blotch discharge printing of tannin-mordant-dyed cotton, as well as in the production of color lakes and inks.
- It is also possible to make a fluorescent variant of the Schiff reagent by using Auramine O.
- It also has antiseptic properties and can be used for that purpose.
FAQs
Q. What fluorescence is emitted by Auramine O?
When exposed to blue light (450–500 nm), Auramine O becomes excited and emits light in the range of around 500 nm to 700 nm (green–yellow–red).
Q. What does Auramine O stain?
Auramine O stain is utilized for the direct detection of mycobacteria in clinical specimens. It binds to mycolic acids and emits light when exposed to ultraviolet light. Acid-fast organisms (mycobacteria) thus appear yellow or orange in the UV light.
Resources
Biological Stains | Classification, Examples & Uses